![]() pseudotuberculosis no earlier than 26,000 years ago ( Achtman et al., 1999 Morelli et al., 2010). Population genetics analysis revealed that Y. pseudotuberculosis are nearly identical at the genomic level, they cause very different diseases. These findings provided the opportunity to uncover virulence-associated genes due to the avirulent nature of 91001 in humans through comparative genomics ( Zhou et al., 2004a). ![]() pestis strain whole genomes, KIM and 91001, were decoded ( Lindler et al., 1998 Perry et al., 1998 Deng et al., 2002 Song et al., 2004). pestis (strain CO92) offered unprecedented opportunities for understanding the virulence traits of this deadly pathogen ( Parkhill et al., 2001). pestis, such as plasmids (pCD1, pMT1, and pPCP1) and chromosomal loci ( pgm locus and pH6 antigen coding genes), have already been identified as virulence-associated even before its genome was decoded ( Perry and Fetherston, 1997). We aim to provide a summary of the applications of omics strategies in studying Yersinia pestis, particularly in revealing its virulence. A multitude of extensive reviews introducing different omics technologies exist ( Morrison et al., 2006 Kolker, 2009 Holmes et al., 2010 Knox, 2010 Mahapatra, 2010 Ning and Lo, 2010 Wild, 2010), and we do not intend to repeatedly introduce these concepts and their related techniques in this review. The word “trans-omics” is also used to describe this type of studies ( Tuohy et al., 2009 Yang et al., 2011b Ogata et al., 2012). Combining omics strategies to elucidate specific features of an organism has become a trend, providing a unique opportunity to gain holistic understanding of the physiological and pathological characterization of the studied organism. The development of high-throughput technologies often yields large datasets that require extensive bioinformatic integration to apply omics in biological research. A detailed discussion on omics can be found in the Wikipedia website ( ). Structural Biology and Molecular Biophysics.Omics is a collective concept of high-throughput studies for understanding life ( Morrison et al., 2006) using the integrative strategies of genomics, proteomics, transcriptomics, and metabolomics, as well as the newly developed omics strategies of RNomics ( Bhattacharjee et al., 2012), lipidomics ( Hartler et al., 2012), kinomics ( Kindrachuk et al., 2012), glycomics ( Turnbull and Sasisekharan, 2010), peptomics ( Olsen et al., 2002), antigenomics ( Levesque et al., 2012), chemomics ( Wang et al., 2008), etc. We are part of the Laboratory of Molecular Biophysics at the University of Oxford. Our research focuses on developing methods for macromolecular crystallography (MX) to enable problems not previously accessible to structure solution to be tackled. This work currently includes studies on 100K and room temperature (RT) radiation damage and the analysis of liquid and crystalline samples by the tehnique of microPIXE (microbeam Proton Induced X-ray Emission). The group also solve protein structures, most recently of arylamine N-acetyltransferase from Mycobacterium tuberculosis.įor more information about the group please visit our "People" page hereĪbove is the cover of the special issue of the Journal of Synchrotron Radiation on X-ray Radiation Damage to Biological Crystalline Samples (March 2015). ![]() The image on the lower right shows the specific damage distribution (red and blue spheres) of the irradiated baterial protein-DNA complex CEsp1396I/DNA. The work and figure was produced by Charlie Bury. ![]() Introduction to Crystallographyįor an introduction into the method of X-ray crystallography please see this animation which Elspeth and Jonny helped to produce with Oxford Sparks. The British Crystallographic Association (BCA) is the UK national association for crystallograpy. It's main aims are the promotion of crystallography in research and the education of young people and others in the methods, possibilities and achievements of crystallographic science. Special Issue of JSR - In March 2015 a special issue of the Journal of Synchrotron Radiation on X-ray Radiation Damage to Biological Crystalline Samples was published. This issue was edited by Elspeth and Martin Weik and features papers by Charlie Bury and Garman Group Alumnus Markus Gerstel.Įlspeth in puerto Rico - The BioXFEL conference took place in Puerto Rico this year and Elspeth was in attendance. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |